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普諾賽HTR-8/Svneo (人絨毛膜滋養(yǎng)層細(xì)胞)

品名: 普諾賽HTR-8/Svneo (人絨毛膜滋養(yǎng)層細(xì)胞)
型號: CL-0599
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產(chǎn)品信息

名稱HTR-8/Svneo (人絨毛膜滋養(yǎng)層細(xì)胞) (暫不出售)
別稱HTR-8/SV neo; HTR-8/SV-neo; HTR8/SVneo; HTR8svn
背景描述The HTR-8/SVneo cell line was derived by transfecting the cells that grew out of chorinic villi explants of human first-trimester placenta with the gene encoding for simian virus 40 large T antigen.
種屬
年齡(性別)女;6-12周胚胎
組織來源胎盤
生長特性貼壁細(xì)胞
細(xì)胞形態(tài)上皮細(xì)胞樣
細(xì)胞類型轉(zhuǎn)化細(xì)胞系
生物安全等級2
生長培養(yǎng)基RPMI-16405% FBS1% P/S
推薦傳代比例1:3-1:5
推薦換液頻率2~3次/周
凍存條件凍存液:55% 基礎(chǔ)培養(yǎng)基+40%FBS+5%DMSO溫度:液氮
培養(yǎng)條件氣相:空氣,95%;CO2,5%溫度:37℃
致瘤性No (tested in soft agar and nude mice).
抗原表達(dá)情況cytokeratin-7+; insulin-like growth factor (IGF)-II+; NDOG-5 (placental alkaline phosphatase (PLAP) antibody) +; proliferating cell nuclear antigen (PCNA)+; human leukocyte antigen framework antigen (W6/32)+; integrin subunits alpha 1, alpha 3, alpha
保藏機(jī)構(gòu)ATCC; CRL-3271


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